Coding

Part:BBa_K3008009

Designed by: Shayan Vahdani   Group: iGEM19_UCSC   (2019-10-16)


CAHS 94205 + FLAG Tag

Tardigrade-specific intrinsically disordered proteins (TDPs) are required for tardigrade desiccation tolerance, and these genes are sufficient to increase desiccation tolerance when expressed in heterologous systems. TDPs form non-crystalline solids (vitrify) when desiccated. The vitrified state is believed to contribute to their ability to protect the organism. This part encodes the same protein as part <a href="https://parts.igem.org/Part:BBa_K2306006">BBa_K2306006</a>, however, this encodes for the native nucleotide sequence. It also includes a FLAG tag for purification purposes This sequence has not been codon-optimized for expression in any organism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None